INTRODUCTION AND JUSTIFICATION
Waxberry (Myrica rubra Sieb.et Zucc.) is a evergreen fruit grown in the subtropical area. Its cultivating species also named as Deep-red (Zhu-hong) in Fujian province or Tree-berry (Shu-mei) in Taiwan province. It has a long-term cultivating history in China and a broad distribution in the provinces located in the south bank of Yangtze River. The total cultivation area of Waxberry in China is about 0.213 million hm² and 1/5 is located in Zhejiang province (57,000 hm²). Waxberry is a unique cultivated fruit tree in China and international market. Waxberry planting plays positive effect on environment. So waxberry has become the first selecting fruit for the slope areas development. Its cultivation area has been extended year by year all over Zhejiang province. Because of poor management especially poor fertilization, waxberry yield in and fertilization efficient these regions is low.
There were relative large differences in the features of nutrient uptake among the different researchers, which may be caused by the differences in the region, waxberry variety, tree age and soil environment and so on. A investigation in 20 sites of 14 counties/cities of Zhejiang province during the period of 1986 to 1987 by Qin Sui-chu showed that the soil environment of waxberry production was commonly located at the natural soils in the hillside, which is belong to the hilly yellow-red earth subgroup, and these soils including yellow- gravel soil, yellow soil, yellow sandy soil were proper to the good growth of waxberry because of its deeper soil layer and lower slopes with contrast to the poor growth and nutrient disorder in the stone-sandy soil. There were large differences in the soil organic matter and available nutrients among the different regions, and the content of available P in soils often less than 5 mg/kg soil.
Thus, research on the optimum fertilizer-application rates and optimum fertilizer-application time by using large scale field experiments will be very important under lack of knowledge about the relationship of waxberry nutrient uptake features and soil environment. Further research about the optimum application time of P, K fertilizers will be necessary.

OBJECTIVES
By using the results of investigation and sample analysis all over the Zhejiang province and previous soil survey data, the authors established the soil nutrient data base, GIS system of soil nutrient in the waxberry production regions, coupling computer module of waxberry fertilization recommendation, nutrient diagnosis module and pest-disease damage control diagnosis module then to establish the GIS system of soil nutrients and the decision-making sustaining system of fertilization recommendation; to study the soil nutrient-supplying ability and nutrient restrict factors in the main waxberry production regions, and the optimum fertilizer-application rates and optimum application time under different soil fertility levels, to explore the relationship of soil testing values and fertilizer application rates and establish the soil-testing fertilization model and computer-fertilization recommendation module in order to give fertilization recommendation according to the soil nutrient information.
To establish the GIS system of soil nutrients and fertilization recommendation decision-making system in the waxberry production
(a) Information system of soil nutrients in the main waxberry production regions of Zhejiang province;
(b) Agricultural information system in the main waxberry production regions of Zhejiang province;
(c) Fertilization recommendation and pest-disease damage control consultation systems
Study on the soil environment conditions in the famous waxberry production bases
The soil environment conditions in the famous waxberry production bases ;
The soil nutrient status in the famous waxberry production bases;
The plant nutrient status in the good-quality waxberry trees
Study on the main restrict factors of soil nutrients in the waxberry production of Zhejiang province
Study on the good-quality and high-yielding fertilization techniques of the famous good-quality waxberry
The fertilization techniques of good-quality and optimum-yielding waxberry;
The optimum fertilizer application rates of waxberry under different soil fertility levels;
The optimum application times of N,P,K and trace-elements fertilizers

MATERIALS AND METHODS
1 The GIS system of soil nutrients and fertilization recommendation decision-making system in the waxberry production
1.1 The GIS system of soil nutrients in the main waxberry production regions of Zhejiang province: spatial data base with the basic unit of county; property data base including soil types, soil texture( physical particle composition), landform, soil pH, soil OM, P, K, Ca, Mg, S, B, Zn, Fe and Mn;
1.2 Agricultural information system in the main waxberry production regions of Zhejiang province: the production area of waxberry, main cultivation varieties, variety’s characteristics and analysis results of variety’s contents;
1.3 Fertilization recommendation consultation systems of waxberry: to establish the soil-testing fertilization recommendation models according to the multiple-site field-experiment’s results and previous research data; and establish the fertilization recommendation module with coupling of soil nutrient information system by using VB language program;
1.4 Pest-disease damage control consultation systems of waxberry; to collect the data of physiological properties, pathological diseases, pest damage’s symptom and its figure, control methods of pest-disease-damage of waxberry then to establish the data base and recommendation system of diagnosis and control of Pest-disease damage of waxberry by using data base consultation language(SQL)
2 Study on the soil environment conditions in the famous good-quality waxberry production bases
2.1 Investigation of the basic soil environment status in the famous good-quality waxberry production bases (to select the good-quality-optimum-yielding, middle-quality-middle-yielding and poor-quality-lower-yielding waxberry production regions for the investigation of soil types and distribution in order to get the basic data of soil environments of famous good-quality waxberry)
Investigation items:
soil properties:
Soil types: the soils will be classified as four levels including soil group, soil subgroup, soil genus and soil species referenced from the technique regulation of soil survey of Zhejiang province.
Soil parent material: parent material and sediment types
Soil layer depth; < 50cm, 50-100 cm, >100cm
Soil particle composition; to collect soil samples and test the particle composition to determine its texture
Soil compaction: to use soil compaction testing instrument to determine the soil compaction
Investigation of landform and landscape：the land form will be classified as plain, hilly land, hill, mountainous land and indicated its slope direction except for plain
Climatic and hydrological conditions: to collect the data of annual/monthly average temperature, annual highest/lowest temperature, annual total accumulated temperature and frost-free time, as well as the temperature properties affected the yield and quality during the key growing stages of famous good-quality waxberry; to collect the data of monthly/annual precipitation rain fall, evaporation and raining intensity as well as the appearing regulation of rainstorm and seasonable drought.
Investigation of soil management levels and waxberry growth status
Soil management: mainly to investigate the status of soil amending, deep plowing, fertilization, alley cultivation, covering and alien soil covering and the soil construction standard of good-quality, high-yielding waxberry garden.
Cultivation and management levels: mainly to investigate the types and rates and times as well as methods of fertilizer application in waxberry production; also to investigate the relationship of fertilizer sources and fertilizer quality with yield and quality of waxberry; also to investigate the present status of irrigation system and drainage system including water source, water quality and irrigation methods.
Growth status of waxberry tree: to investigate the in-site planting density, growth tendency and growth appearance of waxberry; to realize its changes during the different growing stages then to combine the growth status and soil nutrient analysis data in order to show the actual soil nutrient supplement feature.
2.2 Study on the soil fertility characteristics in the famous good-quality waxberry production bases
Based on the basic status of soils, to collect soil samples for the determination of physical and chemical properties of soils; to analysis soil physical particle composition and soil pH, active organic matter, and N, P, K, Ca, Mg, S, B, Zn, Fe, Mn by using ASI method.
2.3 Investigation of plant nutrient status of famous good-quality waxberry
To collect the fruit and leaf samples on the fruiting braches at harvesting stage for the determination of appear characteristics including fruit weight, fruit diagram and fruit color as well as the contents characteristics including total sugar, soluble sugar, acidity, sugar/acid ratio and Vc content, also to analysis the contents of N,P,K and trace element nutrients in the leaves.
3. Study on the restrict factors of soil nutrients in the main waxberry production regions of Zhejiang province
To collect 250 soil samples from the main waxberry production regions of Zhejiang province for the research of main restrict factors of soil nutrients; based on the laboratory studies, to determine the main restrict factors of soil nutrients according to the nutrient uptake features of waxberry.
3.1 Laboratory analysis: to determine soil pH and the contents of active acids, soil OM, soil N、P、K、Ca、Mg、S、B、Zn、Fe、Mn by using ASI method.
3.2 Field validating experiment
Based on the laboratory analysis of soil restrict factors, the field validating experiment will be conducted at the same locations as the sampling sites. The experiment established the OPT treatment and lack of nutrient-restrict-factor treatment; the optimum treatment’s fertilizer application rates will be followed the chemical analysis results of China-Canada laboratory and the results from absorb experiment; the other of restrict nutrients will be followed the experimental design and the others will be same as the optimum treatment; to select the waxberry garden with more than 8-yr-old waxberry trees; also to determine the yield, fruit quality and leaf’s nutrient contents.
4. The integrated fertilization techniques of good-quality and high-yielding waxberry
4.1 Fertilization techniques of good-quality and optimum-yielding waxberry
Based on the soil nutrient restrict factors, to study the fertilization techniques of good-quality and high-yielding waxberry, and combined balanced fertilization of organic manure and chemical fertilizers, essential elements and middle-elements as well as trace-elements fertilizers, then to supply the optimum nutrients for tree growth then to gain good-quality and high-yielding waxberry to reduce the differences of alternate bearing years.
The main indicators of good-quality waxberry should be as following: weight of per fruit, diagram of fruit, and color of fruit, total sugar, soluble sugar, acidity, sugar/acid ratio and Vc contents of the contents; also the fruit rigidity for long-term storage and long-distance transportation.
4.2 The optimum fertilizer application rates of waxberry
Mainly to conduct the field experiments of the optimum application rates of N,P,K fertilizers of waxberry, with 4 N-application levels of N₀，N₁，N₂，N₃, two P-application levels of P₁,P₂ and three K-application levels of K₀，K₁，K₂; The N application rates experiment was conducted under the conditions to meet its P,K requirements and the experiment of K fertilization will be conducted under the conditions to meet its N,P requirements; the combined treatments will be the following 7 treatments: N₀P₁K₁, N₁P₁K₁, N₂P₁K₁ ,N₃P₁K₁, N₂P₁K₀ ,N₂P₁K₂，N₂P₂K₂; Every experiment will be conducted at more than 10 sites through the Zhejiang province, and the replication number will be followed the local conditions, i.e. four replications or no any replication; Before every experiment beginning, the pre-experiments will be conducted and testing the fruit yield and tree’s growing appearance before the normal experiment beginning in order to eliminate the disturbance from the differences among the waxberry trees; the experiments should been conducted two years including the alternate bearing years, and the fertilizer application rates will be determined by the actual growing status of waxberry trees.
4.3 Study on the optimum times of fertilizer application
Mainly to conduct the field experiments of the optimum times of N,P,K fertilizer application, i.e. based on the same application rates of fertilizers, to establish the following treatments: （1）three-time applications of NPK fertilizers（basal+ fruit-expanding+ after harvesting）;（2）two-time applications of NPK fertilizers（after harvesting +spring-branch-growing），（3）two-time applications of NPK fertilizers (basal + fruit-expanding)，（4）once application of NPK fertilizers（after harvesting）,every experiment will be conducted at 2-4 experimental sites located in the different main waxberry production regions.
4.4 Long-term fertilization experiment of waxberry
To establish the long-term experiments conducted in 1-2 main waxberry production regions to study the long-term effects and subsequent effects of applied N,P,K fertilizers, and establish the following treatments:（1）OPT1( applying N,P,K fertilizers for every year); （2）OPT2( applying N,P,K fertilizers in the first year and no applying any fertilizers then)（3）OPT3 ( applying N,P,K fertilizers in the high-yielding year and no-fertilization in the lower-yielding year)（4）OPT4 ( applying N,P,K fertilizers in the lower-yielding year and no-fertilization in the high-yielding year)（5）OPT-N，（6）OPY-P，（7）OPT-K，the long-term experiments will be conducted for 6 years.
4.5 Demonstration of information fertilization techniques of good-quality and high-yielding waxberry production
Based on the field experiments, to suggest the fertilization recommendation in waxberry production by using soil nutrient information system and fertilization recommendation decision-making system then to achieve the large scale demonstration and extension; to establish comparison experiment fields of farmer’s technique with expert’s suggestion, and to broadcast it in the news medium in order to spread its effects.